Biotinylated Proteins (生物素标记蛋白定制服务)

Biotinylated Proteins  (生物素标记蛋白定制服务)

蛋白的定制服务

生物素化是将生物素以共价键连接到分子（如氨基酸或蛋白质）上的过程。由于生物素很小，生物素化通常快速且具有特异性，扰乱分子固有功能的可能性小。

生物素与链霉亲和素或抗生物素蛋白的结合具有*高的亲和力、快速性和高特异性，因此，生物素化被广泛应用于生物医学领域。值得注意的是，生物素化蛋白作为一种分子工具广泛用于生物技术应用。 Avi-Tag 是一种15个氨基酸残基组成的小标签，能被大肠杆菌生物素连接酶BirA 有效生物素化。Avi-Tag可添加在蛋白的N或C端，产生生物素标记蛋白，用于分离蛋白或显微镜下标记。

Biotinylation is the process of covalently attaching biotin to a molecule, such as amino acid or protein. Generally, Biotinylation is rapid, specific and is unlikely to disturb the natural function of the molecule owing to the small size of biotin. Biotinylation is widely used in biomedical sciences since biotin binds to streptavidin/avidin with an extremely high affinity, fast on-rate, and high specificity. Significantly, biotinylated proteins are widely used as a molecular tool in biotechnological applications. Avi-tag is a small synthetic 15-amino-acid peptide which is effectively biotinylated by BirA, the E. coli biotin ligase, in vitro or in vivo. Avi-Tag can be translationally fused at the N or C terminus of proteins to produce biotin-tagged proteins for isolating protein or labeling for microscopy.